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smart-seq2 method  (Illumina Inc)


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    Structured Review

    Illumina Inc smart-seq2 method
    Smart Seq2 Method, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smart-seq2+method/smart+seq2+libraries/med_rxiv__2023__02__10__23285332-59-6-12
    Average 90 stars, based on 1 article reviews
    smart-seq2 method - by Bioz Stars, 2026-10
    90/100 stars

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    Amplification:

    Article Title: Sustained systemic hyaluronan in COVID-19 patients, a 3D-lung model reveals mechanisms of overproduction counteracted by cortisone
    Article Snippet: RNA-seq libraries were prepared using the Smart-seq2 method, and sequenced on an Illumina NextSeq 500 (75PE, v2.5 High Output kit).

    Article Title: Integrative discovery of treatments for high-risk neuroblastoma
    Article Snippet: The RNA-Seq libraries were prepared in a 384-well plate format using the SMART-Seq2 method and sequenced on an Illumina HiSeq 3000 instrument for 51 cycles, targeting around 0.8 million reads per well as an output, which is sufficient to probe the transcriptome at the level of principal components, gene signatures and pathways , .

    Article Title: CD74 regulates cellularity and maturation of medullary thymic epithelial cells partially by activating the canonical NF‐κB signaling pathway
    Article Snippet: © 2021 Federation of American Societies for Experimental Biology HongXia Wang, Qian Zhang, Jiayu Zhang, Rong Luan contributed equally to this work.. Abbreviations: Aire, autoimmune regulator; APCs, antigenpresenting cell; cTEC, cortical thymic epithelial cell; DEGs, differentially expressed genes; DN, double negative; DP, double positive; FACS, fluorescenceactivated cell sorting; FITC, fluorescein isothiocyanate; H&E, hematoxylin and eosin; MHC, major histocompatibility complex; mTEC, medullary thymic epithelial cell; mTOR, mammalian/mechanistic target of rapamycin; MIF, macrophage migration inhibitory factor; mTORC1, mammalian/mechanistic target of rapamycin complex 1; mTORC2, mammalian/mechanistic target of rapamycin complex 2; MFI, mean fluorescence intensity; NFκB, nuclear factor kappalightchainenhancer of activated B cells; RANKL, Receptor Activator for Nuclear FactorκB Ligand; RT, room temperature; SEM, standard error of the mean; TCR, T cell receptor; TEC, thymic epithelial cell; TRA, tissueresistant antigen; Treg, regulatory T cell; WT, wildtype.. Correspondence YuRong Qiu, Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.

    Sequencing:

    Article Title: Sustained systemic hyaluronan in COVID-19 patients, a 3D-lung model reveals mechanisms of overproduction counteracted by cortisone
    Article Snippet: RNA-seq libraries were prepared using the Smart-seq2 method, and sequenced on an Illumina NextSeq 500 (75PE, v2.5 High Output kit).

    Article Title: Integrative discovery of treatments for high-risk neuroblastoma
    Article Snippet: The RNA-Seq libraries were prepared in a 384-well plate format using the SMART-Seq2 method and sequenced on an Illumina HiSeq 3000 instrument for 51 cycles, targeting around 0.8 million reads per well as an output, which is sufficient to probe the transcriptome at the level of principal components, gene signatures and pathways , .

    Article Title: CD74 regulates cellularity and maturation of medullary thymic epithelial cells partially by activating the canonical NF‐κB signaling pathway
    Article Snippet: © 2021 Federation of American Societies for Experimental Biology HongXia Wang, Qian Zhang, Jiayu Zhang, Rong Luan contributed equally to this work.. Abbreviations: Aire, autoimmune regulator; APCs, antigenpresenting cell; cTEC, cortical thymic epithelial cell; DEGs, differentially expressed genes; DN, double negative; DP, double positive; FACS, fluorescenceactivated cell sorting; FITC, fluorescein isothiocyanate; H&E, hematoxylin and eosin; MHC, major histocompatibility complex; mTEC, medullary thymic epithelial cell; mTOR, mammalian/mechanistic target of rapamycin; MIF, macrophage migration inhibitory factor; mTORC1, mammalian/mechanistic target of rapamycin complex 1; mTORC2, mammalian/mechanistic target of rapamycin complex 2; MFI, mean fluorescence intensity; NFκB, nuclear factor kappalightchainenhancer of activated B cells; RANKL, Receptor Activator for Nuclear FactorκB Ligand; RT, room temperature; SEM, standard error of the mean; TCR, T cell receptor; TEC, thymic epithelial cell; TRA, tissueresistant antigen; Treg, regulatory T cell; WT, wildtype.. Correspondence YuRong Qiu, Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.



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